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LearnPA5-89226
antibody from Invitrogen Antibodies
Targeting: NRBF2
COPR1, COPR2, DKFZp564C1664, FLJ30395
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- PA5-89226 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- NRBF2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: LAHQQSRRAD RLLAAGKYEE AISCHKKAAA YLSEAMKLTQ SEQAHLSLEL QRDSHMKQLL LIQERWKRAQ REERLKAQQN TDKDAAAHLQ TSHKPSAEDA EGQSPLSQKY SPSTEKCLPE IQGIFDRDPD TLLYLLQQKS EPAEPCIGSK APKDDKTIIE EQATKIADLK RHVEFLVAEN ERLRKENKQL KAEKARLLKG PIEKELDVDA D; Positive Samples: HepG2, 293T, HeLa, Jurkat; Cellular Location: Cytoplasm, Cytoplasmic vesicle, Nucleus, autophagosome
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 2.38 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of extracts of various cell lines, using NRBF2 Polyclonal antibody (Product # PA5-89226) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Exposure time: 90s.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of NRBF2 in extracts of various cell lines using NRBF2 Polyclonal Antibody (Product # PA5-89226) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of NRBF2 was performed in MCF7 cells using NRBF2 Polyclonal Antibody (Product # PA5-89226). Blue: DAPI for nuclear staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of NRBF2 in paraffin-embedded human liver cancer using NRBF2 Polyclonal Antibody (Product # PA5-89226) at a dilution of 1:100.
