Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [2]
- Immunoprecipitation [2]
- Immunohistochemistry [2]
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Validation data
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- Product number
- NBP1-19128 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-19128, RRID:AB_1642503
- Product name
- Rabbit Polyclonal LARP1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 0.2 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references LARP1 binding to hepatitis C virus particles is correlated with intracellular retention of viral infectivity.
Epidermal Growth Factor Receptor-Dependent Mutual Amplification between Netrin-1 and the Hepatitis C Virus.
The Zinc-Finger Antiviral Protein ZAP Inhibits LINE and Alu Retrotransposition.
Plissonnier ML, Cottarel J, Piver E, Kullolli M, Centonze FG, Pitteri S, Farhan H, Meunier JC, Zoulim F, Parent R
Virus research 2019 Oct 2;271:197679
Virus research 2019 Oct 2;271:197679
Epidermal Growth Factor Receptor-Dependent Mutual Amplification between Netrin-1 and the Hepatitis C Virus.
Plissonnier ML, Lahlali T, Michelet M, Lebossé F, Cottarel J, Beer M, Neveu G, Durantel D, Bartosch B, Accardi R, Clément S, Paradisi A, Devouassoux-Shisheboran M, Einav S, Mehlen P, Zoulim F, Parent R
PLoS biology 2016 Mar;14(3):e1002421
PLoS biology 2016 Mar;14(3):e1002421
The Zinc-Finger Antiviral Protein ZAP Inhibits LINE and Alu Retrotransposition.
Moldovan JB, Moran JV
PLoS genetics 2015 May;11(5):e1005121
PLoS genetics 2015 May;11(5):e1005121
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: LARP1 Antibody [NBP1-19128] - Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-LARP1 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: LARP1 Antibody [NBP1-19128] - Whole cell lysate (15 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-LARP1 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: LARP1 Antibody [NBP1-19128] - Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-LARP1 antibody used for IP at 6 ug per reaction. LARP1 was also immunoprecipitated by rabbit anti-LARP1 antibody A302-088A. For blotting immunoprecipitated LARP1, was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: LARP1 Antibody [NBP1-19128] - Detection of human LARP1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-LARP1 NBP1-19128 used for IP at 3 ug per reaction. LARP1 was also immunoprecipitated by rabbit anti-LARP1 antibody NBP1-19129. For blotting immunoprecipitated LARP1, NBP1-19128 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: LARP1 Antibody [NBP1-19128] - FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-LARP1 used at a dilution of 1:200 (1ug/ml). Detection: DAB
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: LARP1 Antibody [NBP1-19128] - Human colon carcinoma. Antibody: Affinity purified rabbit anti-LARP1 used at a dilution of 1:200 (1ug/ml). Detection: DAB