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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- ELISA [1]
- Immunoprecipitation [1]
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Validation data
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- Product number
- H00093436-M03 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00093436-M03, RRID:AB_1237647
- Product name
- ARMC6 monoclonal antibody (M03), clone 2H8
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against a full-length recombinant ARMC6.
- Antigen sequence
MVSKRIAQETFDAAVRENIEEFAMGPEEAVKEAVE
QFESQGVDLSNIVKTAPKVSADGSQEPTHDILQML
SDLQESVASSRPQEVSAYLTRFCDQCKQDKACRFL
AAQKGAYPIIFTAWKLATAGDQGLLLQSLNALSVL
TDGQPDLLDAQGLQLLVATLTQNADEADLTCSGIR
CVRHACLKHEQNRQDLVKAGVLPLLTGAITHHGHH
TDVVREACWALRVMTFDDDIRVPFGHAHNHAKMIV
QENKGLKVLIEATKAFLDNPGILSELCGTLSRLAI
RNEFCQEVVDLGGLSILVSLLADCNDHQMRDQSGV
QELVKQVLSTLRAIAGNDDVKDAIVRAGGTESIVA
AMTQHLTSPQVCEQSCAALCFLALRKPDNSRIIVE
GGGAVAALQAMKAHPQKAGVQKQACMLIRNLVAHG
QAFSKPILDLGAEALIMQARSAHRDCEDVAKAALR
DLGCHVELRELWTGQRGNLAP- Isotype
- IgG
- Antibody clone number
- 2H8
- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western Blot analysis of ARMC6 expression in transfected 293T cell line by ARMC6 monoclonal antibody (M03), clone 2H8.Lane 1: ARMC6 transfected lysate (Predicted MW: 51.5 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged ARMC6 is 3 ng/ml as a capture antibody.
- Validation comment
- Sandwich ELISA (Recombinant protein)
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunoprecipitation of ARMC6 transfected lysate using anti-ARMC6 monoclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with ARMC6 monoclonal antibody.
- Validation comment
- Immunoprecipitation
- Protocol
- Protocol
