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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Western blot [4]
- Immunohistochemistry [1]
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- Product number
- NBP2-38770 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rabbit Polyclonal PLOD1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. PLOD1 antibody verified on Human Protein Array containing target protein plus 383 other non-specific proteins.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Inhibition of enzymes involved in collagen cross-linking reduces vascular smooth muscle cell calcification.
Sc65-Null Mice Provide Evidence for a Novel Endoplasmic Reticulum Complex Regulating Collagen Lysyl Hydroxylation.
Jover E, Silvente A, Marín F, Martínez-González J, Orriols M, Martinez CM, Puche CM, Valdés M, Rodriguez C, Hernández-Romero D
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2018 Aug;32(8):4459-4469
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2018 Aug;32(8):4459-4469
Sc65-Null Mice Provide Evidence for a Novel Endoplasmic Reticulum Complex Regulating Collagen Lysyl Hydroxylation.
Heard ME, Besio R, Weis M, Rai J, Hudson DM, Dimori M, Zimmerman SM, Kamykowski JA, Hogue WR, Swain FL, Burdine MS, Mackintosh SG, Tackett AJ, Suva LJ, Eyre DR, Morello R
PLoS genetics 2016 Apr;12(4):e1006002
PLoS genetics 2016 Apr;12(4):e1006002
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: PLOD1 Antibody [NBP2-38770] - Analysis using Anti-PLOD1 antibody NBP2-38770 (A) shows similar pattern to independent antibody NBP2-31885 (B).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: PLOD1 Antibody [NBP2-38770] - Analysis in human cell line U-87 MG.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: PLOD1 Antibody [NBP2-38770] - Analysis in human cell line U-251 MG and human cell line RT-4.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: PLOD1 Antibody [NBP2-38770] - SC65 directly interacts with lysyl-hydroxylase 1 (LH1). Western blot of primary calvarial osteoblast and skin fibroblast lysates from WT and Sc65KO 3 day-old mice (N = 2) showing significantly decreased levels of LH1 protein in Sc65KO samples. Densitometric quantification of LH1 protein normalized to beta-actin from the western blot shown above (*p
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: PLOD1 Antibody [NBP2-38770] - Staining of human placenta shows cytoplasmic positivity in decidual cells.
