Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunohistochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- PA5-48414 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-PLOD1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Predicted to react with mouse based on sequence homology.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µl
- Concentration
- Lot Dependent
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of PLOD1 in (Lane 1) A431 whole cell lysates (20 µg), (Lane 2) MCF-7 whole cell lysates (20 µg), (Lane 3) U-2OS whole cell lysates (20 µg), and (Lane 4) U-87 MG whole cell lysates. Samples were probed with a PLOD1 Antibody (N-Term) (Product # PA5-48414) at 1:2000 dilution. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1:10000 dilution. Predicted band size : 84 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of PLOD1 in (Lane 1) MCF-7 whole cell lysates (20 µg), (Lane 2) K562 whole cell lysates (20 µg), and (Lane 3) U-2OS whole cell lysates (20 µg). Samples incubated in a PLOD1 Antibody (N-term) (Product # PA5-48414) at a 1:1000-1:2000 dilution, followed by Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at a 1:10000 dilution. Predicted band size : 84 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of PLOD1 in U251 cell line lysates (35 µg/lane). Samples probed with a PLOD1 Antibody (N-term) (Product # PA5-48414).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of PLOD1 in paraformaldehyde-fixed, paraffin-embedded Human heart tissue sections. Samples were incubated with a PLOD1 antibody (Product # PA5-48414) at a 1:25 dilution for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometric analysis of PLOD 1 in U-87 MG cells. Cells were fixed, permeabilized, inclubated in 2% BSA, and stained with a PLOD1 polyclonal Antibody (Product # PA5-48414, green line) at a 1:25 dilution. Following incubation with the primary antibody for 1 hour at 37°C, cells were incubated with a Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed antibody at 1:400 dilution for 40 min at 37°C. Isotype control antibody (blue line) was mouse IgG1 (1µg/1x10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.