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Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
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- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
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- Product number
- GTX130498 - Provider product page
- Provider
- GeneTex
- Product name
- Gemin 5 antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse
- Host
- Rabbit
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Gemin 5 antibody detects Gemin 5 protein by western blot analysis. Various whole cell extracts (30 ?g) were separated by 5% SDS-PAGE, and the membrane was blotted with Gemin 5 antibody (GTX130498) diluted at a dilution of 1:500.
- Validation comment
- WB
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Gemin 5 antibody detects Gemin 5 protein at cytoplasm and nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Gemin 5 protein stained by Gemin 5 antibody (GTX130498) diluted at 1:500.Blue: Hoechst 33342 staining.Scale bar = 10 £gm.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunoprecipitation of Gemin 5 protein from HepG2 whole cell extracts using 5 £gg of Gemin 5 antibody (GTX130498).Western blot analysis was performed using Gemin 5 antibody (GTX130498).EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
