PAB23104

antibody from Abnova Corporation

Targeting: GEMIN5

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB23104

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#PAB23104, RRID:AB_11118718

Product name

GEMIN5 polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against recombinant GEMIN5.

Antigen sequence

YDSGSFTIMQEVYSAFLPDGCDHLRDKLGDHQSPA
TPAFKSLEAFFLYGRLYEFWWSLSRPCPNSSVWVR
AGHRTLSVEPSQQLDTASTEETDPET


Isotype

IgG

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of Lane 1: RT-4, Lane 2: U-251 MG, Lane 3: Human Plasma, Lane 4: Liver, Lane 5: Tonsil with GEMIN5 polyclonal antibody (Cat # PAB23104) at 1:250-1:500 dilution.

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line A-431 with GEMIN5 polyclonal antibody (Cat # PAB23104) at 1-4 ug/mL dilution shows positivity in nucleus but not nucleoli and cytoplasm.

Validation comment

Immunofluorescence

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical staining of human cerebellum with GEMIN5 polyclonal antibody (Cat # PAB23104) shows strong cytoplasmic and nucleolar positivity in purkinje cells at 1:200-1:500 dilution.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)