24897-1-AP

antibody from Invitrogen Antibodies

Targeting: GEMIN5

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

24897-1-AP

Provider

Invitrogen Antibodies

Product name

GEMIN5 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: AVWKSIFSL DTPEQYQEAF QKLQNIKYPS ATNNTPAKQL LLHICHDLTL AVLSQQMASW DEAVQALLRA VVRSYDSGSF TIMQEVYSAF LPDGCDHLRD KLGDHQSPAT PAFKSLEAFF LYGRLYEFWW SLSRPCPNSS VWVRAGHRTL SVEPSQQLDT ASTEETDPET SQPEPNRPSE LDLRLTEEGE RMLSTFKELF SEKHASLQNS QRTVAEVQET LAEMIRQHQK SQLCKSTANG PDKNEPEVEA EQPLCSSQSQ CKEEKNEPLS LPELTKRLTE ANQRMAKFPE SIKAWPFPDV LECCLVLLLI RSHFPGCLAQ EMQQQAQELL QKYGNTKTYR RHCQTFCM (1162-1508 aa encoded by BC113614)

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.49 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Jurkat cells were subjected to SDS PAGE followed by western blot with 24897-1-AP ( GEMIN5 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HeLa cells were subjected to SDS PAGE followed by western blot with 24897-1-AP (GEMIN5 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HEK-293 cells were subjected to SDS PAGE followed by western blot with 24897-1-AP (GEMIN5 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of HeLa cells using 24897-1-AP (GEMIN5 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IGG.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human cerebellum tissue slide using 24897-1-AP ( GEMIN5 Antibody) at dilution of 1:50 (under 40x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human cerebellum tissue slide using 24897-1-AP (GEMIN5 Antibody) at dilution of 1:50 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human cervical cancer tissue slide using 24897-1-AP (GEMIN5 Antibody) at dilution of 1:50 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human cervical cancer tissue slide using 24897-1-AP (GEMIN5 Antibody) at dilution of 1:50 (under 40x lens).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-GEMIN5 (IP:24897-1-AP, 5ug; Detection:24897-1-AP 1:1000) with HeLa cells lysate 220ug.