710377

antibody from Invitrogen Antibodies

Targeting: MAP3K8 c-COT, COT, EST, ESTF, MEKK8, Tpl-2

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

710377

Provider

Invitrogen Antibodies

Product name

TPL2 Recombinant Polyclonal Antibody (3HCLC)

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

This antibody is predicted to react with bovine, canine, equine, mouse, rabbit and rat based on sequence homology.

Antibody clone number

3HCLC

Concentration

0.5 mg/mL

References

Submitted references

Janus Kinase 1 Plays a Critical Role in Mammary Cancer Progression.

Wehde BL, Rädler PD, Shrestha H, Johnson SJ, Triplett AA, Wagner KU
Cell reports 2018 Nov 20;25(8):2192-2207.e5

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of TPL2 in whole cell extracts from Jurkat cells (lane 1), 3T3L1 cells (lane 2), and HepG2 cells (lane 3) using a TPL2 Recombinant Rabbit Polyclonal Antibody (Product # 710377) at a dilution of 2 µg/mL. Samples were detected using chemiluminescence (ECL). Results show a band at ~52kDa.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of COT/TPL2/MAP3K8 was done on 70% confluent log phase U-87MG cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with COT/TPL2/MAP3K8 (3HCLC), Recombinant Rabbit Polyclonal Antibody (Product # 710377) at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d is a merged image showing cytoplasmic localization. Panel e is a no primary antibody control. The images were captured at 60X magnification.