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ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- MA5-24365 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Ninjurin 1 Monoclonal Antibody (758926)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- Reconstitute in sterile PBS to a final concentration of 0.5 mg/mL.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 758926
- Vial size
- 100 µg
- Concentration
- 0.5 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of Ninjurin 1 in immersion fixed paraffin-embedded sections of human liver. Samples were incubated in Ninjurin 1 monoclonal antibody (Product # MA5-24365) using a dilution of 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown) and counterstained with hematoxylin (blue). Specific staining was localized to Kupffer cells.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry of Ninjurin 1 in HepG2 human hepatocellular carcinoma cell line. Samples were incubated in Ninjurin 1 monoclonal antibody (Product # MA5-24365) or isotype control antibody followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody.
