H00084299-M02

antibody from Abnova Corporation

Targeting: MIEN1 C17orf37, C35, MGC14832, ORB3, Rdx12, XTP4

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Antibody Data

Product number

H00084299-M02

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00084299-M02, RRID:AB_10615760

Product name

C17orf37 monoclonal antibody (M02), clone 3B5

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a full-length recombinant C17orf37.

Antigen sequence

MSGEPGQTSVAPPPEEVEPGSGVRIVVEYCEPCGF
EATYLELASAVKEQYPGIEIESRLGGTGAFEIEIN
GQLVFSKLENGGFPYEKDLIEAIRRASNGETLEKI
TNSRPPCVIL


Isotype

IgG

Antibody clone number

3B5

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

References

Submitted references

MicroRNA-940 suppresses prostate cancer migration and invasion by regulating MIEN1.

Rajendiran S, Parwani AV, Hare RJ, Dasgupta S, Roby RK, Vishwanatha JK
Molecular cancer 2014 Nov 19;13:250

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Prenylated c17orf37 induces filopodia formation to promote cell migration and metastasis.

Dasgupta S, Cushman I, Kpetemey M, Casey PJ, Vishwanatha JK
The Journal of biological chemistry 2011 Jul 22;286(29):25935-46

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of C17orf37 expression in transfected 293T cell line by C17orf37 monoclonal antibody (M02), clone 3B5.Lane 1: C17orf37 transfected lysate(12.4 KDa).Lane 2: Non-transfected lysate.

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged C17orf37 is 0.3 ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol