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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [5]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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Validation data
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- Product number
- GTX101733 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX101733, RRID:AB_2036915
- Product name
- Ferritin Heavy Chain antibody [N1C3]
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ug of whole cell lysate) A: A549 12% SDS PAGE GTX101733 diluted at 1:1000
- Validation comment
- WB
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Human tissue extract (30 ?g) was separated by 12% SDS-PAGE, and the membrane was blotted with Ferritin Heavy Chain antibody [N1C3] (GTX101733) diluted at 1:500.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Mouse tissue extract (50 ?g) was separated by 12% SDS-PAGE, and the membrane was blotted with Ferritin Heavy Chain antibody [N1C3] (GTX101733) diluted at 1:500.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Rat tissue extract (50 ?g) was separated by 12% SDS-PAGE, and the membrane was blotted with Ferritin Heavy Chain antibody [N1C3] (GTX101733) diluted at 1:500.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Whole cell extract (30 ?g) was separated by 12% SDS-PAGE, and the membrane was blotted with Ferritin Heavy Chain antibody [N1C3] (GTX101733) diluted at 1:500.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Ferritin Heavy Chain antibody [N1C3] detects Ferritin Heavy Chain protein at cytoplasm and nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Ferritin Heavy Chain protein stained by Ferritin Heavy Chain antibody [N1C3] (GTX101733) diluted at 1:500.Red: Phalloidin, a cytoskeleton marker, diluted at 1:50.Blue: Hoechst 33342 staining.Scale bar = 10 £gm.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Ferritin Heavy Chain antibody [N1C3] detects Ferritin Heavy Chain protein at cytoplasm in mouse liver by immunohistochemical analysis. Sample: Paraffin-embedded mouse liver. Ferritin Heavy Chain antibody [N1C3] (GTX101733) diluted at 1:500.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Ferritin Heavy Chain antibody [N1C3] detects Ferritin Heavy Chain protein at cytoplasm in rat liver by immunohistochemical analysis. Sample: Paraffin-embedded rat liver. Ferritin Heavy Chain antibody [N1C3] (GTX101733) diluted at 1:500.
