200-401-090S

antibody from Rockland Immunochemicals, Inc.

Targeting: FTH1 FHC, FTH, FTHL6, PIG15, PLIF

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

200-401-090S

Provider

Rockland Immunochemicals, Inc.

Proper citation

Rockland Cat#200-401-090S, RRID:AB_2612113

Product name

Anti-FERRITIN (Human Spleen) (RABBIT) Antibody - 200-401-090S

Antibody type

Polyclonal

Vial size

25 µl

References

Submitted references

Dynamic O-GlcNAcylation coordinates ferritinophagy and mitophagy to activate ferroptosis.

Yu F, Zhang Q, Liu H, Liu J, Yang S, Luo X, Liu W, Zheng H, Liu Q, Cui Y, Chen G, Li Y, Huang X, Yan X, Zhou J, Chen Q
Cell discovery 2022 May 3;8(1):40

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A Combined Model of Human iPSC-Derived Liver Organoids and Hepatocytes Reveals Ferroptosis in DGUOK Mutant mtDNA Depletion Syndrome.

Guo J, Duan L, He X, Li S, Wu Y, Xiang G, Bao F, Yang L, Shi H, Gao M, Zheng L, Hu H, Liu X
Advanced science (Weinheim, Baden-Wurttemberg, Germany) 2021 May;8(10):2004680

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Human Parainfluenza Virus Type 2 V Protein Modulates Iron Homeostasis.

Ohta K, Saka N, Nishio M
Journal of virology 2021 Feb 24;95(6)

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Potential Point-of-Care Microfluidic Devices to Diagnose Iron Deficiency Anemia.

Yap BK, M Soair SN, Talik NA, Lim WF, Mei I L
Sensors (Basel, Switzerland) 2018 Aug 10;18(8)

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Autophagy-Independent Lysosomal Targeting Regulated by ULK1/2-FIP200 and ATG9.

Goodwin JM, Dowdle WE, DeJesus R, Wang Z, Bergman P, Kobylarz M, Lindeman A, Xavier RJ, McAllister G, Nyfeler B, Hoffman G, Murphy LO
Cell reports 2017 Sep 5;20(10):2341-2356

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Salinomycin kills cancer stem cells by sequestering iron in lysosomes.

Mai TT, Hamaï A, Hienzsch A, Cañeque T, Müller S, Wicinski J, Cabaud O, Leroy C, David A, Acevedo V, Ryo A, Ginestier C, Birnbaum D, Charafe-Jauffret E, Codogno P, Mehrpour M, Rodriguez R
Nature chemistry 2017 Oct;9(10):1025-1033

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Dedicated SNAREs and specialized TRIM cargo receptors mediate secretory autophagy.

Kimura T, Jia J, Kumar S, Choi SW, Gu Y, Mudd M, Dupont N, Jiang S, Peters R, Farzam F, Jain A, Lidke KA, Adams CM, Johansen T, Deretic V
The EMBO journal 2017 Jan 4;36(1):42-60

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Ferritinophagy via NCOA4 is required for erythropoiesis and is regulated by iron dependent HERC2-mediated proteolysis.

Mancias JD, Pontano Vaites L, Nissim S, Biancur DE, Kim AJ, Wang X, Liu Y, Goessling W, Kimmelman AC, Harper JW
eLife 2015 Oct 5;4

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Selective VPS34 inhibitor blocks autophagy and uncovers a role for NCOA4 in ferritin degradation and iron homeostasis in vivo.

Dowdle WE, Nyfeler B, Nagel J, Elling RA, Liu S, Triantafellow E, Menon S, Wang Z, Honda A, Pardee G, Cantwell J, Luu C, Cornella-Taracido I, Harrington E, Fekkes P, Lei H, Fang Q, Digan ME, Burdick D, Powers AF, Helliwell SB, D'Aquin S, Bastien J, Wang H, Wiederschain D, Kuerth J, Bergman P, Schwalb D, Thomas J, Ugwonali S, Harbinski F, Tallarico J, Wilson CJ, Myer VE, Porter JA, Bussiere DE, Finan PM, Labow MA, Mao X, Hamann LG, Manning BD, Valdez RA, Nicholson T, Schirle M, Knapp MS, Keaney EP, Murphy LO
Nature cell biology 2014 Nov;16(11):1069-79

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Quantitative proteomics identifies NCOA4 as the cargo receptor mediating ferritinophagy.

Mancias JD, Wang X, Gygi SP, Harper JW, Kimmelman AC
Nature 2014 May 1;509(7498):105-9

Western blot

Supportive validation

Submitted by

Rockland Immunochemicals, Inc. (provider)

Main image

Experimental details

Western Blot of Rabbit Anti-Ferritin antibody. Lane 1: Ferritin. Lane 2: None. Load: 50 ng per lane. Primary antibody: Ferritin primary antibody at 1:1,000 overnight at 4°C. Secondary antibody: Peroxidase rabbit secondary antibody at 1:40,000 for 30 min at RT. Blocking: MB-070 for 30 min at RT. Predicted/Observed size: 20 kDa, 20 kDa for Ferritin. Other band(s): None.

Validation comment

Western Blot