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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [4]
- Immunocytochemistry [2]
- Other assay [1]
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Validation data
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- Product number
- 13691-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- HERC4 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: NIDYKKPLK VIFVGEDAVD AGGVRKEFFL LIMRELLDPK YGMFRYYEDS RLIWFSDKTF EDSDLFHLIG VICGLAIYNC TIVDLHFPLA LYKKLLKKKP SLDDLKELMP DVGRSMQQLL DYPEDDIEET FCLNFTITVE NFGATEVKEL VLNGADTAVN KQNRQEFVDA YVDYIFNKSV ASLFDAFHAG FHKVCGGKVL LLFQPNELQA MVIGNTNYDW KELEKNTEYK GEYWAEHPTI KIFWEVFHEL PLEKKKQFLL FLTGSDRIPI LGMKSLKLVI QSTGGGEEYL PVSHTCFNLL DLPKYTEKET LRSKLIQAID HNEGFSLI (723-1049 aa encoded by BC039600)
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.27 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HeLa cells were subjected to SDS PAGE followed by western blot with 13691-1-AP (HERC4 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Human testis tissue were subjected to SDS PAGE followed by western blot with 13691-1-AP (HERC4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Human brain tissue were subjected to SDS PAGE followed by western blot with 13691-1-AP (HERC4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Human placenta tissue were subjected to SDS PAGE followed by western blot with 13691-1-AP (HERC4 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using HERC4 antibody (Product # 13691-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of HeLa cells using 13691-1-AP (HERC4 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-HERC4 (IP:13691-1-AP, 4ug; Detection:13691-1-AP 1:800) with HeLa cells lysate 2000ug.
