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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [16]
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- NBP1-25959 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-25959, RRID:AB_1726016
- Product name
- Mouse Monoclonal Palladin Antibody
- Antibody type
- Monoclonal
- Description
- Protein G purified.
- Reactivity
- Human, Mouse, Rat, Canine, Chicken/Avian
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 1 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Palladin Compensates for the Arp2/3 Complex and Supports Actin Structures during Listeria Infections.
Exosomal microRNAs derived from colorectal cancer-associated fibroblasts: role in driving cancer progression.
Coordinated Regulation of Palladin and α-Smooth Muscle Actin by Transforming Growth Factor-β in Human Corneal Fibroblasts.
Autophagy protects cardiomyocytes from the myocardial ischaemia-reperfusion injury through the clearance of CLP36.
Dual roles of palladin protein in in vitro myogenesis: inhibition of early induction but promotion of myotube maturation.
Palladin is upregulated in kidney disease and contributes to epithelial cell migration after injury.
Aberrant gene expression in dogs with portosystemic shunts.
Arousal of cancer-associated stroma: overexpression of palladin activates fibroblasts to promote tumor invasion.
Isoform-specific upregulation of palladin in human and murine pancreas tumors.
Palladin contributes to invasive motility in human breast cancer cells.
Human adipose-derived adult stem cells upregulate palladin during osteogenesis and in response to cyclic tensile strain.
Movement of stress fibers away from focal adhesions identifies focal adhesions as sites of stress fiber assembly in stationary cells.
Identification of palladin isoforms and characterization of an isoform-specific interaction between Lasp-1 and palladin.
Palladin binds to Eps8 and enhances the formation of dorsal ruffles and podosomes in vascular smooth muscle cells.
Characterization of palladin, a novel protein localized to stress fibers and cell adhesions.
Characterization of palladin, a novel protein localized to stress fibers and cell adhesions.
Dhanda AS, Vogl AW, Albraiki SE, Otey CA, Beck MR, Guttman JA
mBio 2018 Apr 10;9(2)
mBio 2018 Apr 10;9(2)
Exosomal microRNAs derived from colorectal cancer-associated fibroblasts: role in driving cancer progression.
Bhome R, Goh RW, Bullock MD, Pillar N, Thirdborough SM, Mellone M, Mirnezami R, Galea D, Veselkov K, Gu Q, Underwood TJ, Primrose JN, De Wever O, Shomron N, Sayan AE, Mirnezami AH
Aging 2017 Dec 28;9(12):2666-2694
Aging 2017 Dec 28;9(12):2666-2694
Coordinated Regulation of Palladin and α-Smooth Muscle Actin by Transforming Growth Factor-β in Human Corneal Fibroblasts.
Morishige N, Murata S, Nakamura Y, Azumi H, Shin-Gyou-Uchi R, Oki KT, Morita Y, Sonoda KH
Investigative ophthalmology & visual science 2016 Jun 1;57(7):3360-8
Investigative ophthalmology & visual science 2016 Jun 1;57(7):3360-8
Autophagy protects cardiomyocytes from the myocardial ischaemia-reperfusion injury through the clearance of CLP36.
Li S, Liu C, Gu L, Wang L, Shang Y, Liu Q, Wan J, Shi J, Wang F, Xu Z, Ji G, Li W
Open biology 2016 Aug;6(8)
Open biology 2016 Aug;6(8)
Dual roles of palladin protein in in vitro myogenesis: inhibition of early induction but promotion of myotube maturation.
Nguyen NU, Wang HV
PloS one 2015;10(4):e0124762
PloS one 2015;10(4):e0124762
Palladin is upregulated in kidney disease and contributes to epithelial cell migration after injury.
Chang EH, Gasim AH, Kerber ML, Patel JB, Glaubiger SA, Falk RJ, Jennette JC, Otey CA
Scientific reports 2015 Jan 9;5:7695
Scientific reports 2015 Jan 9;5:7695
Aberrant gene expression in dogs with portosystemic shunts.
van Steenbeek FG, Van den Bossche L, Grinwis GC, Kummeling A, van Gils IH, Koerkamp MJ, van Leenen D, Holstege FC, Penning LC, Rothuizen J, Leegwater PA, Spee B
PloS one 2013;8(2):e57662
PloS one 2013;8(2):e57662
Arousal of cancer-associated stroma: overexpression of palladin activates fibroblasts to promote tumor invasion.
Brentnall TA, Lai LA, Coleman J, Bronner MP, Pan S, Chen R
PloS one 2012;7(1):e30219
PloS one 2012;7(1):e30219
Isoform-specific upregulation of palladin in human and murine pancreas tumors.
Goicoechea SM, Bednarski B, Stack C, Cowan DW, Volmar K, Thorne L, Cukierman E, Rustgi AK, Brentnall T, Hwang RF, McCulloch CA, Yeh JJ, Bentrem DJ, Hochwald SN, Hingorani SR, Kim HJ, Otey CA
PloS one 2010 Apr 26;5(4):e10347
PloS one 2010 Apr 26;5(4):e10347
Palladin contributes to invasive motility in human breast cancer cells.
Goicoechea SM, Bednarski B, García-Mata R, Prentice-Dunn H, Kim HJ, Otey CA
Oncogene 2009 Jan 29;28(4):587-98
Oncogene 2009 Jan 29;28(4):587-98
Human adipose-derived adult stem cells upregulate palladin during osteogenesis and in response to cyclic tensile strain.
Wall ME, Rachlin A, Otey CA, Loboa EG
American journal of physiology. Cell physiology 2007 Nov;293(5):C1532-8
American journal of physiology. Cell physiology 2007 Nov;293(5):C1532-8
Movement of stress fibers away from focal adhesions identifies focal adhesions as sites of stress fiber assembly in stationary cells.
Endlich N, Otey CA, Kriz W, Endlich K
Cell motility and the cytoskeleton 2007 Dec;64(12):966-76
Cell motility and the cytoskeleton 2007 Dec;64(12):966-76
Identification of palladin isoforms and characterization of an isoform-specific interaction between Lasp-1 and palladin.
Rachlin AS, Otey CA
Journal of cell science 2006 Mar 15;119(Pt 6):995-1004
Journal of cell science 2006 Mar 15;119(Pt 6):995-1004
Palladin binds to Eps8 and enhances the formation of dorsal ruffles and podosomes in vascular smooth muscle cells.
Goicoechea S, Arneman D, Disanza A, Garcia-Mata R, Scita G, Otey CA
Journal of cell science 2006 Aug 15;119(Pt 16):3316-24
Journal of cell science 2006 Aug 15;119(Pt 16):3316-24
Characterization of palladin, a novel protein localized to stress fibers and cell adhesions.
Parast MM, Otey CA
The Journal of cell biology 2000 Aug 7;150(3):643-56
The Journal of cell biology 2000 Aug 7;150(3):643-56
Characterization of palladin, a novel protein localized to stress fibers and cell adhesions.
Parast MM, Otey CA
The Journal of cell biology 2000 Aug 7;150(3):643-56
The Journal of cell biology 2000 Aug 7;150(3):643-56
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Palladin Antibody (1E6) [NBP1-25959] - MDA-MB-231 lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Palladin Antibody (1E6) [NBP1-25959] - Western blot analysis of HeLa (A), A431 (B), NIH3T3 (C), and PC-12 (D) cell line extracts using Palladin antibody at 1ug/ml.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Palladin Antibody (1E6) [NBP1-25959] - Palladin was detected in immersion fixed paraffin-embedded sections of human heart using Mouse Anti-Human Palladin (1E6) Monoclonal Antibody (Catalog # NBP1-25959) at 1:100 for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm in cardiomyocytes.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: Palladin Antibody (1E6) [NBP1-25959] - An intracellular stain was performed on HeLa cells with Palladin Antibody [1E6] NBP1-25959AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.
