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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [2]
- Protein array [1]
- Other assay [1]
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- Product number
- V3862 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- Mammaglobin A Antibody / SCGB2A2
- Antibody type
- Monoclonal
- Description
- Protein G affinity chromatography
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antibody clone number
- MGB1/2000
- Vial size
- 20 µg, 100 µg (with BSA, sodium azide), 100 µg (without BSA, sodium azide), 7 ml (prediluted for IHC)
- Concentration
- 0.2 mg/ml, 1 mg/ml (BSA/azide free format), Prediluted (IHC only format)
- Storage
- Store the Mammaglobin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of human SK-BR-3 cell lysate (mammary gland tumor cell line) with Mammaglobin antibody (clone MGB1/2000). Expected molecular weight: 10-21 kDa depending on level of glycosylation.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC testing of FFPE human breast carcinoma stained with Mammaglobin antibody (MGB1/2000). Required HIER: boiling tissue sections in 10mM citrate buffer, pH6, for 10-20 min followed by cooling at RT for 20 min.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC testing of FFPE human breast carcinoma stained with Mammaglobin antibody (MGB1/2000). Required HIER: boiling tissue sections in 10mM citrate buffer, pH6, for 10-20 min followed by cooling at RT for 20 min.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using Mammaglobin antibody (clone MGB1/2000). These results demonstrate the foremost specificity of the MGB1/2000 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SDs) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SDs) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- SDS-PAGE analysis of purified, BSA-free Mammaglobin antibody (clone MGB1/2000) as confirmation of integrity and purity.
