Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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Validation data
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- Product number
- 18002-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PP11 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: MRACISLVL AVLCGLAWAE DHKESEPLPQ LEEETEEALA SNLYSAPTSC QGRCYEAFDK HHQCHCNARC QEFGNCCKDF ESLCSDHEVS HSSDAITKEE IQSISEKIYR ADTNKAQKED IVLNSQNCIS PSETRNQVDR CPKPLFTYVN EKLFSKPTYA AFINLLNNYQ RATGHGEHFS AQELAEQDAF LREIMKTAVM KELYSFLHHQ NRYGSEQEFV DDLKNMWFGL YSRGNEEGDS SGFEHVFSGE VKKGKVTGFH NWIRFYLEEK EGLVDYYSHI YDGPWDSYPD VLAMQFNWDG YYKEVGSAFI GSSPEFEFAL YSLCFIARPG KVCQLSLGGY PLAVRTYTWD KSTYGNGKKY IATAYIVSST (1-369 aa encoded by BC074763)
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.23 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse lung tissue were subjected to SDS PAGE followed by western blot with 18002-1-AP (PP11 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HeLa cells were subjected to SDS PAGE followed by western blot with 18002-1-AP (PP11 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of ( -20°C Ethanol ) fixed HeLa cells using 18002-1-AP (PP11 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 18002-1-AP (PP11 antibody) at dilution of 1:200 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 18002-1-AP (PP11 antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).