Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [3]
- Other assay [1]
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Validation data
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- Product number
- PA5-35859 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ATOH7 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Role of mTORC1 activity during early retinal development and lamination in human-induced pluripotent stem cell-derived retinal organoids.
Lee SH, Han JW, Yang JY, Jun HO, Bang JH, Shin H, Choi JH, Lee J, Madrakhimov SB, Chung KH, Chang HS, Lyu J, Park TK
Cell death discovery 2022 Feb 8;8(1):56
Cell death discovery 2022 Feb 8;8(1):56
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- ATOH7 Polyclonal Antibody detects ATOH7 protein by western blot analysis. A. 30 µg 293T whole cell lysate/extract. B. 30 µg whole cell lysate/extract of human ATOH7-transfected 293T cells.15 % SDS-PAGE. ATOH7 Polyclonal Antibody (Product # PA5-35859) dilution: 1:10,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- ATOH7 Polyclonal Antibody detects ATOH7 protein at cytosol and nucleus on mouse colon by immunohistochemical analysis. Sample: Paraffin-embedded mouse colon. ATOH7 Polyclonal Antibody (Product # PA5-35859) dilution: 1:1,000. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- ATOH7 Polyclonal Antibody detects ATOH7 protein at cytosol on mouse intestine by immunohistochemical analysis. Sample: Paraffin-embedded mouse intestine. ATOH7 Polyclonal Antibody (Product # PA5-35859) dilution: 1:2,500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- ATOH7 Polyclonal Antibody detects ATOH7 protein at cytosol on mouse intestine by immunohistochemical analysis. Sample: Paraffin-embedded mouse intestine. ATOH7 Polyclonal Antibody (Product # PA5-35859) dilution: 1:2,500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig. 1 Formation of hiPSC-derived ROs and RGC development at an early stage. A Main steps of hiPSC-derived RO development in vitro: hiPSC colony, EB formation, neuroretinal domain, and ROs. B Immunofluorescence staining of HuC/D (green) and AtoH7 (red) of ROs at distinct differentiated time points. The expression of HuC/D and AtoH7 was gradually increased in the innermost layers of ROs from day 35 to day 60. C Quantitative RT-PCR results of PAX6, AtoH7, Islet-1, Brn3b, and Tuj1 levels of ROs at indicated time points. Data are expressed by mean +- S.E.M. * p < 0.05, ** p < 0.01 by the Kruskal-Wallis test with post hoc analysis. Scale bar, 50 mum.