Antibody data
- Antibody Data
- Antigen structure
- References [6]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- NB100-57086 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-57086, RRID:AB_843835
- Product name
- Goat Polyclonal DGAT1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse
- Host
- Goat
- Antigen sequence
QNSMKPFKDMDYS
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 0.5 mg/ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
Submitted references Analysis of the liver lipidome reveals insights into the protective effect of exercise on high-fat diet-induced hepatosteatosis in mice.
Lipidomic and spatio-temporal imaging of fat by mass spectrometry in mice duodenum during lipid digestion.
Caspase-1 deficiency in mice reduces intestinal triglyceride absorption and hepatic triglyceride secretion.
Skeletal muscle triglycerides, diacylglycerols, and ceramides in insulin resistance: another paradox in endurance-trained athletes?
Adipose triglyceride lipase in human skeletal muscle is upregulated by exercise training.
Overexpression of diacylglycerol acyltransferase-1 reduces phospholipid synthesis, proliferation, and invasiveness in simian virus 40-transformed human lung fibroblasts.
Jordy AB, Kraakman MJ, Gardner T, Estevez E, Kammoun HL, Weir JM, Kiens B, Meikle PJ, Febbraio MA, Henstridge DC
American journal of physiology. Endocrinology and metabolism 2015 May 1;308(9):E778-91
American journal of physiology. Endocrinology and metabolism 2015 May 1;308(9):E778-91
Lipidomic and spatio-temporal imaging of fat by mass spectrometry in mice duodenum during lipid digestion.
Seyer A, Cantiello M, Bertrand-Michel J, Roques V, Nauze M, Bézirard V, Collet X, Touboul D, Brunelle A, Coméra C
PloS one 2013;8(4):e58224
PloS one 2013;8(4):e58224
Caspase-1 deficiency in mice reduces intestinal triglyceride absorption and hepatic triglyceride secretion.
van Diepen JA, Stienstra R, Vroegrijk IO, van den Berg SA, Salvatori D, Hooiveld GJ, Kersten S, Tack CJ, Netea MG, Smit JW, Joosten LA, Havekes LM, van Dijk KW, Rensen PC
Journal of lipid research 2013 Feb;54(2):448-56
Journal of lipid research 2013 Feb;54(2):448-56
Skeletal muscle triglycerides, diacylglycerols, and ceramides in insulin resistance: another paradox in endurance-trained athletes?
Amati F, Dubé JJ, Alvarez-Carnero E, Edreira MM, Chomentowski P, Coen PM, Switzer GE, Bickel PE, Stefanovic-Racic M, Toledo FG, Goodpaster BH
Diabetes 2011 Oct;60(10):2588-97
Diabetes 2011 Oct;60(10):2588-97
Adipose triglyceride lipase in human skeletal muscle is upregulated by exercise training.
Alsted TJ, Nybo L, Schweiger M, Fledelius C, Jacobsen P, Zimmermann R, Zechner R, Kiens B
American journal of physiology. Endocrinology and metabolism 2009 Mar;296(3):E445-53
American journal of physiology. Endocrinology and metabolism 2009 Mar;296(3):E445-53
Overexpression of diacylglycerol acyltransferase-1 reduces phospholipid synthesis, proliferation, and invasiveness in simian virus 40-transformed human lung fibroblasts.
Bagnato C, Igal RA
The Journal of biological chemistry 2003 Dec 26;278(52):52203-11
The Journal of biological chemistry 2003 Dec 26;278(52):52203-11
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: DGAT1 Antibody [NB100-57086] - Staining of Rat Duodenum lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: DGAT1 Antibody [NB100-57086] - Staining of Mouse Liver lysate (35 ug protein in RIPA buffer). Antibody at 0.03 ug/mL. Detected by chemiluminescence.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: DGAT1 Antibody [NB100-57086] - Staining of paraffin embedded Human Colon. Antibody at 3.75 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: DGAT1 Antibody [NB100-57086] - Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.