A305-177A

antibody from Invitrogen Antibodies

Targeting: ZNF451 COASTER, dJ417I1.1, KIAA0576, KIAA1702

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Recommended by provider)

Antibody Data

Product number

A305-177A

Provider

Invitrogen Antibodies

Product name

ZNF451 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1 mg/mL

Storage

4° C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human ZNF451 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-ZNF451 antibody A305-177A (lot A305-177A-1) used for IP at 6 µg per reaction. ZNF451 was also immunoprecipitated by rabbit anti-ZNF451 antibody BL20399. For blotting immunoprecipitated ZNF451, A305-177A was used at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human ZNF451 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, 293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-ZNF451 antibody A305-177A (lot A305-177A-1) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.