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LearnA305-177A
antibody from Invitrogen Antibodies
Targeting: ZNF451
COASTER, dJ417I1.1, KIAA0576, KIAA1702
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
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- Product number
- A305-177A - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ZNF451 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- The recommended shelf life for this product is 1 year from date of receipt.
- Concentration
- 1 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human ZNF451 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-ZNF451 antibody A305-177A (lot A305-177A-1) used for IP at 6 µg per reaction. ZNF451 was also immunoprecipitated by rabbit anti-ZNF451 antibody BL20399. For blotting immunoprecipitated ZNF451, A305-177A was used at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human ZNF451 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, 293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-ZNF451 antibody A305-177A (lot A305-177A-1) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
