Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- GTX130828 - Provider product page
- Provider
- GeneTex
- Product name
- Nek1 antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse
- Host
- Rabbit
No comments: Submit comment
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Whole cell extract (30 £gg) was separated by 5% SDS-PAGE, and the membrane was blotted with Nek1 antibody (GTX130828) diluted at 1:1000.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Various tissue extracts (50 £gg) were separated by 5% SDS-PAGE, and the membrane was blotted with Nek1 antibody (GTX130828) diluted at 1:1000.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Nek1 antibody detects Nek1 protein at cytoplasm and nucleus by immunofluorescent analysis.Sample: U87-MG cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Nek1 protein stained by Nek1 antibody (GTX130828) diluted at 1:500.Blue: Hoechst 33342 staining.Scale bar = 10 £gm.
