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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [5]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- PAB3282 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB3282, RRID:AB_1577640
- Product name
- NEK1 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of NEK1.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references The master cell cycle regulator APC-Cdc20 regulates ciliary length and disassembly of the primary cilium.
Nek1 and TAZ interact to maintain normal levels of polycystin 2.
Identification of proteins that interact with the central coiled-coil region of the human protein kinase NEK1.
Antigens recognized by autologous antibody in patients with renal-cell carcinoma.
A mammalian dual specificity protein kinase, Nek1, is related to the NIMA cell cycle regulator and highly expressed in meiotic germ cells.
Wang W, Wu T, Kirschner MW
eLife 2014 Aug 19;3:e03083
eLife 2014 Aug 19;3:e03083
Nek1 and TAZ interact to maintain normal levels of polycystin 2.
Yim H, Sung CK, You J, Tian Y, Benjamin T
Journal of the American Society of Nephrology : JASN 2011 May;22(5):832-7
Journal of the American Society of Nephrology : JASN 2011 May;22(5):832-7
Identification of proteins that interact with the central coiled-coil region of the human protein kinase NEK1.
Surpili MJ, Delben TM, Kobarg J
Biochemistry 2003 Dec 30;42(51):15369-76
Biochemistry 2003 Dec 30;42(51):15369-76
Antigens recognized by autologous antibody in patients with renal-cell carcinoma.
Scanlan MJ, Gordan JD, Williamson B, Stockert E, Bander NH, Jongeneel V, Gure AO, Jäger D, Jäger E, Knuth A, Chen YT, Old LJ
International journal of cancer 1999 Nov 12;83(4):456-64
International journal of cancer 1999 Nov 12;83(4):456-64
A mammalian dual specificity protein kinase, Nek1, is related to the NIMA cell cycle regulator and highly expressed in meiotic germ cells.
Letwin K, Mizzen L, Motro B, Ben-David Y, Bernstein A, Pawson T
The EMBO journal 1992 Oct;11(10):3521-31
The EMBO journal 1992 Oct;11(10):3521-31
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- The NEK1 polyclonal antibody (Cat # PAB3282) is used in Western blot to detect NEK1 in CHO cell lysate.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with NEK1 polyclonal antibody (Cat # PAB3282) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. HC = hepatocarcinoma.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
