Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PAB23481 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB23481, RRID:AB_11121199
- Product name
- NEK1 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against recombinant NEK1.
- Antigen sequence
VLKEQLERKRKEAYEREKKVWEEHLVAKGVKSSDV
SPPLGQHETGGSPSKQQMRSVISVTSALKEVGVDS
SLTDTRETSEEMQKTNNAISSKR- Isotype
- IgG
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescent staining of human cell line U-251 MG with NEK1 polyclonal antibody (Cat # PAB23481) at 1-4 ug/mL dilution shows positivity in nuclear membrane.
- Validation comment
- Immunofluorescence
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunohistochemical staining of human breast with NEK1 polyclonal antibody (Cat # PAB23481) shows strong cytoplasmic and membranous positivity in glandular cells at 1:200-1:500 dilution.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)