Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
- Blocking/Neutralizing [1]
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Validation data
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- Product number
- AF7340 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Sheep Polyclonal Laminin alpha 4 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human Laminin alpha 4 in direct ELISAs and Western blots. In direct ELISAs, approximately 15% cross-reactivity with recombinant mouse Laminin alpha 4 is observed.
- Reactivity
- Human
- Host
- Sheep
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references Monoclonal antibodies to human laminin α4 chain globular domain inhibit tumor cell adhesion and migration on laminins 411 and 421, and binding of α6β1 integrin and MCAM to α4-laminins.
Ishikawa T, Wondimu Z, Oikawa Y, Ingerpuu S, Virtanen I, Patarroyo M
Matrix biology : journal of the International Society for Matrix Biology 2014 Jun;36:5-14
Matrix biology : journal of the International Society for Matrix Biology 2014 Jun;36:5-14
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Human Laminin alpha 4 by Western Blot. Western blot shows lysates of human placenta tissue. PVDF membrane was probed with 2 µg/mL of Sheep Anti-Human Laminin alpha 4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7340) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Laminin alpha 4 at approximately 200-220 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Human Laminin a4 by Simple WesternTM. Detection of Human Laminin a4 by Simple WesternTM.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Laminin a4 in Human Placenta. Laminin a4 in Human Placenta.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Adhesion Induced by Laminin alpha 4 and Neutralization by Human Laminin alpha 4 Antibody. Recombinant Human Laminin alpha 4 (Catalog # 7340-A4) supports adhesion in the HT1080 human fibrosarcoma cell line in a dose-dependent manner (orange line), as measured by Calcein AM. Adhesion elicited by Recombinant Human Laminin alpha 4 (5 µg/mL) is neutralized (green line) by increasing concentrations of Sheep Anti-Human Laminin alpha 4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7340). The ND50 is typically 1.5-7.5 µg/mL.