Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Other assay [1]
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Validation data
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- Product number
- PA5-38938 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-Laminin alpha-4 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- -20°C
Submitted references Chronic High Glucose Concentration Induces Inflammatory and Remodeling Changes in Valvular Endothelial Cells and Valvular Interstitial Cells in a Gelatin Methacrylate 3D Model of the Human Aortic Valve.
Ciortan L, Macarie RD, Cecoltan S, Vadana M, Tucureanu MM, Mihaila AC, Droc I, Butoi E, Manduteanu I
Polymers 2020 Nov 25;12(12)
Polymers 2020 Nov 25;12(12)
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Laminin alpha-4/LAMA4 in extracts from COLO cells and 293 cells using a Laminin alpha-4/LAMA4 polyclonal antibody (Product # PA5-38938).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Laminin alpha-4/LAMA4 in NIH-3T3 cells using a Laminin alpha-4/LAMA4 polyclonal antibody (Product # PA5-38938).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of Laminin alpha-4/LAMA4 in paraffin-embedded human heart tissue using a Laminin alpha-4/LAMA4 polyclonal antibody (Product # PA5-38938).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 6 ( A ) Gene expression of extracellular matrix (ECM) proteins and MMPs (MMP-1 and MMP-13) in VEC and VIC cultured in 3D in HG versus NG glucose exposure, as evaluated by real-time PCR. The mRNA of investigated molecules was normalized to actin mRNA. The results are presented as fold change versus NG condition for every type of cell and investigated time. N = 3, * p < 0.5, ** p < 0.01. ( B ) Identification of ECM protein in 3D constructs with VEC and VIC, grown in NG or HG for 7 and 14 days. Representative images of laminin alpha4 chain and collagen III in sections obtained from 3D constructs grown in NG and HG for 7 and 14 days. The images were taken with an 10x objective, with filter for DAPI (nuclei-cyan) and filter Alexa 594 (laminin and collagen III-red).