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Western blot
Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- ELISA [1]
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- Product number
- H00005600-M03 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00005600-M03, RRID:AB_829448
- Product name
- Mouse Monoclonal p38 beta/MAPK11 Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. MAPK11 (1F9)
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: p38 beta/MAPK11 Antibody (1F9) [H00005600-M03] - Western blot analysis of MAPK11 over-expressed 293 cell line, cotransfected with MAPK11 Validated Chimera RNAi or non-transfected control. Blot probed with H00005600-M03. GAPDH (36.1 kDa) used as loading control.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: p38 beta/MAPK11 Antibody (1F9) [H00005600-M03] - Analysis of MAPK11 expression in transfected 293T cell line by MAPK11 monoclonal antibody (M03), clone 1F9. Lane 1: MAPK11 transfected lysatE (41.4 KDa). Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: p38 beta/MAPK11 Antibody (1F9) [H00005600-M03] - Detection limit for recombinant GST tagged MAPK11 is approximately 10ng/ml as a capture antibody.
