PA5-14050

antibody from Invitrogen Antibodies

Targeting: MAPK11 p38-2, p38Beta, PRKM11, SAPK2

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-14050

Provider

Invitrogen Antibodies

Product name

p38 MAPK beta Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

400 µL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

Interacting post-muscarinic receptor signaling pathways potentiate matrix metalloproteinase-1 expression and invasion of human colon cancer cells.

Said AH, Hu S, Abutaleb A, Watkins T, Cheng K, Chahdi A, Kuppusamy P, Saxena N, Xie G, Raufman JP
The Biochemical journal 2017 Feb 20;474(5):647-665

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis using a MAPK11 polyclonal antibody (Product # PA5-14050) in mouse brain tissue lysate. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of formalin-fixed, paraffin-embedded human cancer tissue using a MAPK11 polyclonal antibody (Product # PA5-14050), followed by HRP-conjugated secondary antibody and AEC staining.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 7 Effects of p38-alpha knockdown on ACh- and PMA-induced MMP1 expression ( A ) HT-29 cells were transfected with Lipofectamine (L), 50 pmoles non-targeting mock siRNA (siR-M), or 50 pmoles siRNA targeting p38-alpha (siR-p38-alpha). p38-alpha, p38-beta, total p38, and beta-actin expression were measured by immunoblotting with specific antibodies. ( B ) HT-29 cells transfected with Lipofectamine (control), non-targeting mock siRNA, and siRNA targeting p38-alpha were incubated with saline, 100 muM ACh, or 50 nM PMA for 4 h at 37degC. MMP1 mRNA levels were measured by qPCR. qPCR data were normalized to GAPDH and are means of four separate experiments. * P < 0.05; *** P < 0.001.