Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunohistochemistry [2]
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Validation data
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- Product number
- 12493-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SGEF Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: MISFLILPM QRVTRLPLLM DTICQKTPKD SPKYEVCKRA LKEVSKLVRL CNEGARKMER TEMMYTINSQ LEFKIKPFPL VSSSRWLVKR GELTAYVEDT VLFSRRTSKQ QVYFFLFKDV LIITKKKSEE SYNVNDYSLR DQLLVESCDN EELNSSPGKN SSTMLYSRQS SASHLFTLTV LSNHANEKVE MLLGAETQSE RARWITALGH SSGKPPADRT SLTQVEIVRS FTAKQPDELS LQVADVVLIY QRVSDGWYEG ERLRDGERGW FPMECAKEIT CQATIDKNVE RMGRLLGLET NV (1-301 aa encoded by BC016628)
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.14 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HeLa cells were subjected to SDS PAGE followed by western blot with 12493-1-AP (SGEF antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- PC-3 cells were subjected to SDS PAGE followed by western blot with 12493-1-AP (SGEF antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- PC-3 cells were subjected to SDS PAGE followed by western blot with 12493-1-AP (SGEF antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human prostate cancer tissue slide using 12493-1-AP ( SGEF antibody) at dilution of 1:200 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human prostate cancer tissue slide using 12493-1-AP ( SGEF antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).