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Learn10470-1-AP
antibody from Invitrogen Antibodies
Targeting: CLINT1
CLINT, ENTH, EPNR, KIAA0171
Western blot Immunocytochemistry
Immunoprecipitation Immunohistochemistry Flow cytometry Other assayAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
- Flow cytometry [1]
- Other assay [1]
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- Product number
- 10470-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CLINT1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: LVDLFDGTS QSTGGSADLF GGFADFGSAA ASGSFPSQVT ATSGNGDFGD WSAFNQAPSG PVASSGEFFG SASQPAVELV SGSQSALGPP PAASNSSDLF DLMGSSQATM TSSQSMNFSM MSTNTVGLGL PMSRSQNTDM VQKSVSKTLP STWSDPSVNI SLDNLLPGMQ PSKPQQPSLN TMIQQQNMQQ PMNVMTQSFG AVNLSSPSNM LPVRPQTNAL IGGPMPMSMP NVMTGTMGMA PLGNTPMMNQ SMMGMNMNIG MSAAGMGLTG TMGMGMPNIA MTSGTVQPKQ DAFANFANFS K (326-625 aa encoded by BC004467)
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.3 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HepG2 cells were subjected to SDS PAGE followed by western blot with 10470-1-AP (CLINT1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HeLa cells were subjected to SDS PAGE followed by western blot with 10470-1-AP (CLINT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of HepG2 cells, using CLINT1antibody 10470-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IGG (red).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 10470-1-AP ( CLINT1 Antibody) at dilution of 1:200 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 10470-1-AP ( CLINT1 Antibody) at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- 1X10^6 HepG2 cells were stained with 0.2ug CLINT1 antibody (10470-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L) with dilution 1:1000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-CLINT1 (IP:10470-1-AP, 4ug; Detection:10470-1-AP 1:1000) with HeLa cells lysate 2800ug.
