MA5-29497

antibody from Invitrogen Antibodies

Targeting: PPM1G PP2CG, PP2Cgamma

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-29497

Provider

Invitrogen Antibodies

Product name

PPM1G Recombinant Rabbit Monoclonal Antibody (112)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Description

This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%).

Antibody clone number

112

Concentration

1 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of PPM1G in Lane A: MCF7 Whole Cell lysate (30 µg), Lane B: Hela Whole Cell lysate (30 µg). Samples were probed using a PPM1G Monoclonal Antibody (Product # MA5-29497) at a 1:200 dilution, followed by a Goat Anti-Rabbit IgG (H+L), Dylight 800 Secondary Antibody at a 1:10000 dilution. Western blot was performed under reducing conditions. Predicted band size:22 kDa. Observed band size:22 kDa.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using PPM1G Recombinant Rabbit Monoclonal Antibody (112) (Product # MA5-29497) at 1:500 dilution. Lane A: Hela Whole Cell Lysate, Lane B: 293T Whole Cell Lysate, Lane C: Jurkat Whole Cell Lysate. Lysates/proteins at 30 μg per lane. Secondary antibody: Goat Anti-Rabbit IgG H&L (DyLight™ 800) at 1:10,000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 59 kDa.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence staining of Human PPM1G in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with PPM1G Recombinant Rabbit Monoclonal Antibody (112) (Product # MA5-29497, 1:60) at 4°C overnight. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-rabbit IgG secondary antibody (green).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human PPM1G in human epididymis with PPM1G Recombinant Rabbit Monoclonal Antibody (112) (Product # MA5-29497, 1:200, formalin-fixed paraffin embedded sections). Positive staining was localized to skeletal muscle.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human PPM1G in human heart with PPM1G Recombinant Rabbit Monoclonal Antibody (112) (Product # MA5-29497, 1:200, formalin-fixed paraffin embedded sections). Positive staining was localized to cardiac muscle.