A301-472A

antibody from Invitrogen Antibodies

Targeting: MED24 CRSP100, CRSP4, DRIP100, KIAA0130, MED5, THRAP4, TRAP100

Western blot (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

A301-472A

Provider

Invitrogen Antibodies

Product name

TRAP100/MED24 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

The recommended shelf life for this product is 1 year from date of receipt.

Concentration

1 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human TRAP100/MED24 by western blot. Samples: Whole cell lysate (50 µg) from RKO, GaMG, HEK293T, LNCaP, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-TRAP100/MED24 antibody (Product # A301-472A lot 2) used for WB at 1 µg/mL. Detection: Chemiluminescence with an exposure time of 3 minutes.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of mouse TRAP100/MED24 by western blot. Samples: Whole cell lysate (25 µg) from NIH 3T3, CH27, TCMK-1, and BW5147.3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-TRAP100/MED24 antibody (Product # A301-472A lot 2) used for WB at 1 µg/mL. Detection: Chemiluminescence with an exposure time of 3 minutes.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human and mouse TRAP100/MED24 by western blot (h&m) and immunoprecipitation (h). Samples: Whole cell lysate (50 µg for WB) from MCF7 (7), PANC1 (P), 293T (T) and mouse NIH3T3 (M) cells. For IP, whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells was used. Antibody: Affinity purified rabbit anti-TRAP100/MED24 antibody A301-472A used for WB at 1 µg/ml (A and B) and used for IP at 3 µg/mg lysate. Detection: Chemiluminescence with exposure times of 30 seconds (A and B).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human TRAP100/MED24 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 10% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-TRAP100/MED24 antibody (Product # A301-472A; Lot 2) used for IP at 6 µg per reaction. TRAP100/MED24 was also immunoprecipitated by a previous lot of this antibody (Product # A301-472A; Lot 1). For blotting immunoprecipitated TRAP100/MED24, Product # A301-472A was used at 1 µg/mL. Detection: Chemiluminescence with an exposure time of 30 seconds.