NBP2-59379

antibody from Novus Biologicals

Targeting: CLCN3 ClC-3, CLC3

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Data presented on provider website)

Antibody Data

Product number

NBP2-59379

Provider

Novus Biologicals

Product name

Mouse Monoclonal CLCN3 Antibody

Antibody type

Monoclonal

Description

Protein G purified. Detects 90kDa. Does not cross-react with Clcn4 or Clcn5 (based on KO validation results).

Reactivity

Human, Mouse, Rat

Host

Mouse

Isotype

IgG

Vial size

100 ug

Storage

Store at -20C.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: CLCN3 Antibody (S258-5) [NBP2-59379] - Analysis of Rat Brain Membrane showing detection of ~90 kDa CIcn3 protein using Mouse Anti-CIcn3 Monoclonal Antibody, Clone S258-5. Lane 1: Molecular Weight Ladder. Lane 2: Rat Brain Membrane. Load: 15 ug . Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-CIcn3 Monoclonal Antibody at 1:200 for 16 hours at 4 degrees C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 1 hour RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~90 kDa.