MA5-27709

antibody from Invitrogen Antibodies

Targeting: CLCN3 ClC-3, CLC3

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Recommended by provider)

Antibody Data

Product number

MA5-27709

Provider

Invitrogen Antibodies

Product name

CLCN3 Monoclonal Antibody (S258-5)

Antibody type

Monoclonal

Antigen

Synthetic peptide

Reactivity

Human, Mouse, Rat

Host

Mouse

Isotype

IgG

Antibody clone number

S258-5

Vial size

100 µg

Concentration

1 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of CLCN3 in rat brain membrane with 15 µg of sample. The sample was blocked with 2% BSA and 2% skim milk in TBST, incubated with CLCN3 monoclonal antibody (Product # MA5-27709) using a dilution of 1:200 (16 hours at RT), followed by Goat Anti-Mouse HRP (1 hour at RT) at a dilution of 1:1000 and ECL development (6 min at RT). Samples were arranged as follows: Lane 1: Molecular Weight Ladder, Lane 2: Rat Brain membrane.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of CLCN3 in human neuroblastoma cell line (SK-N-BE). Sample was fixed with 4% formaldehyde (15 min at RT), incubated with CLCN3 monoclonal antibody (Product # MA5-27709) using a dilution of 1:100 (1 hour at RT), and followed by Goat Anti-Mouse 488, Phalloidin Texas Red and DAPI secondary antibody at a dilution of 1:100, 1:1000 (60 min at RT) and 1:5000 (5 min at RT). Images are shown as follows: (A) DAPI (blue) nuclear stain, B) Phalloidin Texas Red F-Actin stain, C) Clcn3 Antibody, D) Merged image. Magnification: 60x.