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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- ELISA [1]
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- Product number
- H00001879-M02 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00001879-M02, RRID:AB_464197
- Product name
- EBF1 monoclonal antibody (M02), clone 1G8
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against a full length recombinant EBF1.
- Antigen sequence
MFGIQESIQRSGSSMKEEPLGSGMNAVRTWMQGAG
VLDANTAAQSGVGLARAHFEKQPPSNLRKSNFFHF
VLALYDRQGQPVEIERTAFVGFVEKEKEANSEKTN
NGIHYRLQLLYSNGIRTEQDFYVRLIDSMTKQAIV
YEGQDKNPEMCRVLLTHEIMCSRCCDKKSCGNRNE
TPSDPVIIDRFFSKFFLKCNQNCLKNAGNPRDMRR
FQVVVSTTVNVDGHVLAVSDNMFVHNNSKHGRRAR
RLDPSEGTPSYLEHATPCIKAISPSEGWTTGGATV
IIIGDNFFDGLQVIFGTMLVWSELITPHAIRVQTP
PRHIPGVVEVTLSYKSKQFCKGTPGRFIYTALNEP
TIDYGFQRLQKVIPRHPGDPERLPKEVILKRAADL
VEALYGMPHNNQEIILKRAADIAEALYSVPRNHNQ
LPALANTSVHAGMMGVNSFSGQLAVNVSEASQATN
QGFTRNSSSVSPHGYVPSTTPQQTNYNSVTTSMNG
YGSAAMSNLGGSPTFLNGSAANSPYAIVPSSPTMA
SSTSLPSNCSSSSGIFSFSPANMVSAVKQKSAFAP
VVRPQTSPPPTCTSTNGNSLQAISGMIVPPM- Isotype
- IgG
- Antibody clone number
- 1G8
- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
Submitted references The Blk pathway functions as a tumor suppressor in chronic myeloid leukemia stem cells.
Zhang H, Peng C, Hu Y, Li H, Sheng Z, Chen Y, Sullivan C, Cerny J, Hutchinson L, Higgins A, Miron P, Zhang X, Brehm MA, Li D, Green MR, Li S
Nature genetics 2012 Jul 15;44(8):861-71
Nature genetics 2012 Jul 15;44(8):861-71
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western Blot analysis of EBF1 expression in transfected 293T cell line by EBF1 monoclonal antibody (M02), clone 1G8.Lane 1: EBF1 transfected lysate(64 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged EBF1 is approximately 0.1ng/ml as a capture antibody.
- Validation comment
- Sandwich ELISA (Recombinant protein)
- Protocol
- Protocol
