Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
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Validation data
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- Product number
- 14159-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- EBF1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: IQESIQRSG SSMKEEPLGS GMNAVRTWMQ GAGVLDANTA AQSGVGLARA HFEKQPPSNL RKSNFFHFVL ALYDRQGQPV EIERTAFVGF VEKEKEANSE KTNNGIHYRL QLLYSNGIRT EQDFYVRLID SMTKQAIVYE GQDKNPEMCR VLLTHEIMCS RCCDKKSCGN RNETPSDPVI IDRFFLKFFL KCNQNCLKNA GNPRDMRRFQ VVVSTTVNVD GHVLAVSDNM FVHNNSKHGR RARRLDPSEG TPSYLEHATP CIKAISPSEG WTTGGATVII IGDNFFDGLQ VIFGTMLVWS ELITPHAIRV QTPPRHIPGV VEVTLSYKSK QFCKGTPGRF IYTALNEP (4-350 aa encoded by BC038805)
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.14 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- 3T3-L1 cells were subjected to SDS PAGE followed by western blot with 14159-1-AP ( EBF1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Raji cells were subjected to SDS PAGE followed by western blot with 14159-1-AP (EBF1 Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using (Product # 14159-1-AP) (EBF1 antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).