Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- ABIN951936 - Provider product page
- Provider
- antibodies-online
- Product name
- anti-DNA Replication Helicase 2 Homolog (Yeast) (DNA2) (AA 337-366), (Middle Region) antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide - KLH conjugated - corresponding to the central region (between 337-366aa) of human DNA2.
- Description
- Purified through a Protein A column followed by peptide affinity purification
- Reactivity
- Human
- Host
- Rabbit
- Epitope
- AA 337-366,Middle Region
- Vial size
- 0.4 mL
- Concentration
- 0.25 mg/mL
- Storage
- Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
- Handling
- Avoid repeated freezing and thawing.
Submitted references Acetylation of Dna2 endonuclease/helicase and flap endonuclease 1 by p300 promotes DNA stability by creating long flap intermediates.
Dna2 on the road to Okazaki fragment processing and genome stability in eukaryotes.
Role of macrophage oxidative burst in the action of anthrax lethal toxin.
Balakrishnan L, Stewart J, Polaczek P, Campbell JL, Bambara RA
The Journal of biological chemistry 2010 Feb 12;285(7):4398-404
The Journal of biological chemistry 2010 Feb 12;285(7):4398-404
Dna2 on the road to Okazaki fragment processing and genome stability in eukaryotes.
Kang YH, Lee CH, Seo YS
Critical reviews in biochemistry and molecular biology 2010 Apr;45(2):71-96
Critical reviews in biochemistry and molecular biology 2010 Apr;45(2):71-96
Role of macrophage oxidative burst in the action of anthrax lethal toxin.
Hanna PC, Kruskal BA, Ezekowitz RA, Bloom BR, Collier RJ
Molecular medicine (Cambridge, Mass.) 1994 Nov;1(1):7-18
Molecular medicine (Cambridge, Mass.) 1994 Nov;1(1):7-18
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Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Western blot analysis in K562 cell line lysates (35ug/lane) using DNA2 antibody Cat.-No. AP51282PU-N. This demonstrates this antibody detected the DNA2 protein (arrow).
Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis in human thymus carcinoma (Formalin-fixed, Paraffin-embedded) using DNA2 antibody Cat.-No. AP51282PU-N, followed by peroxidase conjugated secondary antibody and DAB staining. This data demonstrates the use of the DNA2 antibody for IHC. Clinical relevance has not been evaluated.
Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram) using DNA2 antibody Cat.-No. AP51282PU-N, followed by FITC-conjugated goat-anti-rabbit secondary antibodies.