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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
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- Product number
- TA319372 - Provider product page
- Provider
- OriGene
- Product name
- Rabbit polyclonal anti-CD97 antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal anti-CD97 antibody
- Host
- Rabbit
- Conjugate
- Unconjugated
- Epitope
- Adgre5
- Isotype
- IgG
- Antibody clone number
- NULL
- Vial size
- 500 µg
- Concentration
- 5.0 mg/mL
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- WB using anti-CD97 antibody shows detection of bands corresponding to free Fc-CD97- (5EGF) (lower arrowhead) and Fc-CD97- (5EGF) present as a complex (upper arrowhead) in lysates from COS cells. The left lane contains lysate from cells transfected with control DNA. The right lane contains lysate from COS cells expressing Fc-CD97- (5EGF). The formation of the CD97 complex is currently under investigation. A 1:1,000 dilution of the primary antibody was used.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunofluorescence Microscopy using Protein A purified anti-CD97 antibody shows staining of Fc-CD97-(5EGF) (panel D) in transfected COS cells. Panel A and C shows similar staining using pre-immune serum. Panel A and B show staining of mock transfected COS cells (no vector). A 1:2,500 dilution of the primary antibody was used. Personal Communication. Yvona Ward. NIH, NCI, CCR, Bethesda, MD.
- Validation comment
- IF
