Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [4]
- Immunohistochemistry [3]
- Flow cytometry [1]
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Validation data
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- Product number
- 13071-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD97 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: QDSRGCARW CPQNSSCVNA TACRCNPGFS SFSEIITTPT ETCDDINECA TPSKVSCGKF SDCWNTEGSY DCVCSPGYEP VSGAKTFKNE SENTCQDVDE CSSGQHQCDS STVCFNTVGS YSCRCRPGWK PRHGIPNNQK DTVCEDMTFS TWTPPPGVHS QTLSRFFDKV QDLGRDSKTS SAEVTIQNVI KLVDELMEAP GDVEALAPPV RHLIATQLLS NLEDIMRILA KSLPKGPFTY ISPSNTELTL MIQERGDKNV TMGQSSARMK LNWAVAAGAE DPGPAVAGIL SIQNMTTLLA NASLNLHSKK QAELEEIYES SIRGVQLRRL S (21-350 aa encoded by BC026690)
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.2 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Jurkat cells were subjected to SDS PAGE followed by western blot with 13071-1-AP (CD97 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- U-937 cells were subjected to SDS PAGE followed by western blot with 13071-1-AP (CD97 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- K-562 cells were subjected to SDS PAGE followed by western blot with 13071-1-AP (CD97 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Various lysates were subjected to SDS PAGE followed by western blot with 13071-1-AP (CD97 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human lymphoma using 13071-1-AP (CD97 antibody) at dilution of 1:100 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human tonsillitis tissue slide using 13071-1-AP (CD97 antibody) at dilution of 1:1000 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human tonsillitis tissue slide using 13071-1-AP (CD97 antibody) at dilution of 1:1000 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- 1X10^6 K-562 cells were stained with 0.2ug CD97 antibody (13071-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L) with dilution 1:1500.