AP09133PU-N

antibody from Acris Antibodies GmbH

Targeting: ADGRE5 CD97, TM7LN1

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

AP09133PU-N

Provider

Acris Antibodies GmbH

Proper citation

Acris Antibodies GmbH Cat#AP09133PU-N, RRID:AB_2035338

Product name

anti CD97 (1-512)

Antibody type

Polyclonal

Antigen

Recombinant protein corresponding to amino acids 1-512 (extracellular domain) of Mouse CD97 protein.

Reactivity

Mouse

Host

Rabbit

Isotype

IgG

Vial size

0.5 mg

Concentration

5.0 mg/ml (UV absorbance at 280 nm)

Western blot

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Western blot using Protein A purified anti-CD97 antibody shows detection of bands corresponding to free Fc-CD97- (5EGF) (lower arrowhead) and Fc-CD97- (5EGF) present as a complex (upper arrowhead) in lysates from COS cells. The left lane contains lysate from cells transfected with control DNA. The right lane contains lysate from COS cells expressing Fc-CD97- (5EGF). No staining was noted from bone marrow lysates taken from CD97 knockout mice. The identity of the band at ~65 kDa appearing in all lanes is not known. The formation of the CD97 complex is currently under investigation. Approximately 10 µl of lysate was used in each lane. A 1:1,000 dilution of the primary antibody was used. The image was processed using a 10-sec exposure.

Immunocytochemistry

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Immunofluorescence Microscopy using Protein A purified anti-CD97 antibody shows staining of Fc-CD97-(5EGF) (panel D) in transfected COS cells. Panel A and C shows similar staining using pre-immune serum. Panel A and B show staining of mock transfected COS cells (no vector). A 1:1,500 dilution of the primary antibody was used.