PA5-34916

antibody from Invitrogen Antibodies

Targeting: SLU7 9G8

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-34916

Provider

Invitrogen Antibodies

Product name

SLU7 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Recommended positive controls: 293T, A431, HeLa, HepG2.

Concentration

1 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Slu7 using 30 µg of A) Non-transfected 293T lysates and B) human slu7 transfected 293T lysates. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a Slu7 polyclonal antibody (Product # PA5-34916) at a dilution of 1:5000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using SLU7 Polyclonal Antibody (Product # PA5-34916). Sample (30 µg of whole cell lysate). Lane A: 293T. Lane B: A431. Lane C: HeLa. Lane D: HepG2 . 7.5% SDS PAGE. SLU7 Polyclonal Antibody (Product # PA5-34916) diluted at 1:5,000.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of Slu7 showing staining in the nucleus of HeLa cells. HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min and stained using a Slu7 polyclonal antibody (Product # PA5-34916) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10µm.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of SLU7 was performed in HeLa cells fixed in 4% paraformaldehyde at RT for 15 min. Green: SLU7 Polyclonal Antibody (Product # PA5-34916) diluted at 1:500. Red: phalloidin, a cytoskeleton marker. Blue: Hoechst 33342 staining. Scale bar = 10 µm.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Slu7 antibody detects Slu7 protein at nucleus by immunofluorescent analysis. Sample: HCT116 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Slu7 protein stained by Slu7 antibody (Product # PA5-34916) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 µm.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Slu7 antibody detects SLU7 protein at nucleus on BT483 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded BT483 xenograft. Slu7 antibody (Product # PA5-34916) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.