Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [3]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PA5-28513 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SLU7 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T transfected SLU7 recombinant protein.
- Concentration
- 1 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using SLU7 Polyclonal Antibody (Product # PA5-28513). Sample (30 µg of whole cell lysate). Lane A: Non-transfected 293T lysates. Lane B: hSLU7 transfected 293T lysates. 7.5% SDS PAGE. SLU7 Polyclonal Antibody (Product # PA5-28513) diluted at 1:10,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of SLU7 showing staining in the cytoplasm and nucleus of HeLa cells. HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min and stained using a SLU7 polyclonal antibody (Product # PA5-28513) diluted at 1:1000. Blue: Hoechst 33342 staining. Scale bar = 10µm.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- SLU7 Polyclonal Antibody detects SLU7 protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: SLU7 protein stained by SLU7 Polyclonal Antibody (Product # PA5-28513) diluted at 1:1,000. Blue: Hoechst 33342 staining. Scale bar = 10 µm.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- SLU7 Polyclonal Antibody detects SLU7 protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: SLU7 protein stained by SLU7 Polyclonal Antibody (Product # PA5-28513) diluted at 1:1,000. Blue: Hoechst 33342 staining. Scale bar = 10 µm.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human lung adenocarcinoma, using SLU7 (Product # PA5-28513) antibody at 1:250 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.