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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- PAB4702 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB4702, RRID:AB_1575771
- Product name
- HAND2 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of HAND2.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references A GATA-dependent right ventricular enhancer controls dHAND transcription in the developing heart.
Heart and extra-embryonic mesodermal defects in mouse embryos lacking the bHLH transcription factor Hand1.
Molecular cloning of the human HAND2 gene.
McFadden DG, Charité J, Richardson JA, Srivastava D, Firulli AB, Olson EN
Development (Cambridge, England) 2000 Dec;127(24):5331-41
Development (Cambridge, England) 2000 Dec;127(24):5331-41
Heart and extra-embryonic mesodermal defects in mouse embryos lacking the bHLH transcription factor Hand1.
Firulli AB, McFadden DG, Lin Q, Srivastava D, Olson EN
Nature genetics 1998 Mar;18(3):266-70
Nature genetics 1998 Mar;18(3):266-70
Molecular cloning of the human HAND2 gene.
Russell MW, Kemp P, Wang L, Brody LC, Izumo S
Biochimica et biophysica acta 1998 Dec 22;1443(3):393-9
Biochimica et biophysica acta 1998 Dec 22;1443(3):393-9
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of HAND2 polyclonal antibody (Cat # PAB4702) in NCI-H460 cell lysate (35 ug/lane). HAND2 (arrow) was detected using the purified polyclonal antibody.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with HAND2 polyclonal antibody (Cat # PAB4702) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. HC = hepatocarcinoma.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
