NBP1-31546

Diebold BA, Wilder SG, De Deken X, Meitzler JL, Doroshow JH, McCoy JW, Zhu Y, Lambeth JD
Methods in molecular biology (Clifton, N.J.) 2019;1982:191-229

A novel combinatorial technique for simultaneous quantification of oxygen radicals and aggregation reveals unexpected redox patterns in the activation of platelets by different physiopathological stimuli.

Vara D, Cifuentes-Pagano E, Pagano PJ, Pula G
Haematologica 2019 Sep;104(9):1879-1891

Inositol-triphosphate 3-kinase B confers cisplatin resistance by regulating NOX4-dependent redox balance.

Pan C, Jin L, Wang X, Li Y, Chun J, Boese AC, Li D, Kang HB, Zhang G, Zhou L, Chen GZ, Saba NF, Shin DM, Magliocca KR, Owonikoko TK, Mao H, Lonial S, Kang S
The Journal of clinical investigation 2019 May 13;129(6):2431-2445

Growth of breast cancer cells by leptin is mediated via activation of the inflammasome: Critical roles of estrogen receptor signaling and reactive oxygen species production.

Raut PK, Kim SH, Choi DY, Jeong GS, Park PH
Biochemical pharmacology 2019 Mar;161:73-88

Experimental supporting data on evaluation of skeletal muscle perfusion in canine hind limb ischemia model using color-coded digital subtraction angiography.

Wang T, Su H, Lou W, Gu J, He X, Chen L, Chen G, Song J, Shi W, Zee C, Chen BT
Data in brief 2019 Aug;25:103737

How Supraphysiological Oxygen Levels in Standard Cell Culture Affect Oxygen-Consuming Reactions.

Stuart JA, Fonseca J, Moradi F, Cunningham C, Seliman B, Worsfold CR, Dolan S, Abando J, Maddalena LA
Oxidative medicine and cellular longevity 2018;2018:8238459

Anti-inflammatory and anti-apoptotic effects of rosuvastatin by regulation of oxidative stress in a dextran sulfate sodium-induced colitis model.

Shin SK, Cho JH, Kim EJ, Kim EK, Park DK, Kwon KA, Chung JW, Kim KO, Kim YJ
World journal of gastroenterology 2017 Jul 7;23(25):4559-4568

Cilostazol and enzymatically modified isoquercitrin attenuate experimental colitis and colon cancer in mice by inhibiting cell proliferation and inflammation.

Kangawa Y, Yoshida T, Maruyama K, Okamoto M, Kihara T, Nakamura M, Ochiai M, Hippo Y, Hayashi SM, Shibutani M
Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association 2017 Feb;100:103-114

Peroxiredoxin 6 (Prdx6) supports NADPH oxidase1 (Nox1)-based superoxide generation and cell migration.

Kwon J, Wang A, Burke DJ, Boudreau HE, Lekstrom KJ, Korzeniowska A, Sugamata R, Kim YS, Yi L, Ersoy I, Jaeger S, Palaniappan K, Ambruso DR, Jackson SH, Leto TL
Free radical biology & medicine 2016 Jul;96:99-115

Amyloid β-induced astrogliosis is mediated by β1-integrin via NADPH oxidase 2 in Alzheimer's disease.

Wyssenbach A, Quintela T, Llavero F, Zugaza JL, Matute C, Alberdi E
Aging cell 2016 Dec;15(6):1140-1152

c-Myb regulates NOX1/p38 to control survival of colorectal carcinoma cells.

Pekarčíková L, Knopfová L, Beneš P, Šmarda J
Cellular signalling 2016 Aug;28(8):924-36

NADPH oxidase 4 induces cardiac fibrosis and hypertrophy through activating Akt/mTOR and NFκB signaling pathways.

Zhao QD, Viswanadhapalli S, Williams P, Shi Q, Tan C, Yi X, Bhandari B, Abboud HE
Circulation 2015 Feb 17;131(7):643-55

Neuronal NAD(P)H oxidases contribute to ROS production and mediate RGC death after ischemia.

Dvoriantchikova G, Grant J, Santos AR, Hernandez E, Ivanov D
Investigative ophthalmology & visual science 2012 May 14;53(6):2823-30

NOX5 in human spermatozoa: expression, function, and regulation.

Musset B, Clark RA, DeCoursey TE, Petheo GL, Geiszt M, Chen Y, Cornell JE, Eddy CA, Brzyski RG, El Jamali A
The Journal of biological chemistry 2012 Mar 16;287(12):9376-88

Nickel-induced epithelial-mesenchymal transition by reactive oxygen species generation and E-cadherin promoter hypermethylation.

Wu CH, Tang SC, Wang PH, Lee H, Ko JL
The Journal of biological chemistry 2012 Jul 20;287(30):25292-302

Changes in the nasopharyngeal carcinoma nuclear proteome induced by the EBNA1 protein of Epstein-Barr virus reveal potential roles for EBNA1 in metastasis and oxidative stress responses.

Cao JY, Mansouri S, Frappier L
Journal of virology 2012 Jan;86(1):382-94

Ammonia reduction with ornithine phenylacetate restores brain eNOS activity via the DDAH-ADMA pathway in bile duct-ligated cirrhotic rats.

Balasubramaniyan V, Wright G, Sharma V, Davies NA, Sharifi Y, Habtesion A, Mookerjee RP, Jalan R
American journal of physiology. Gastrointestinal and liver physiology 2012 Jan 1;302(1):G145-52

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Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Western Blot: NOX1 Antibody [NBP1-31546] - Sample (30 ug of whole cell lysate) A: JC B: BCL-1 7. 5% SDS PAGE; antibody diluted at 1:5000.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Western Blot: NOX1 Antibody [NBP1-31546] - Sample (30 ug of whole cell lysate) A: 293T B: A431 C: HeLa 7. 5% SDS PAGE, antibody diluted at 1:2000.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Western Blot: NOX1 Antibody [NBP1-31546] - Representative Western blots showing Nox1 and beta-tubulin in PC-3 cells or Nox4 and beta-tubulin in C2C12 cells, at 5% and 18% O2. Total cellular proteins were extracted by treating cells with NP-40 buffer (150mM NaCl, 1% NP-40, 50mM Tris-HCl pH8.0). Total protein (15ug per sample) was resolved on 10% SDS-PAGE, transferred to a polyvinylidene difluoride membrane, and probed for Nox1 or Nox4. beta-Tubulin was used as an internal loading and transfer control. All antibodies were purchased from Novus Biologicals; Nox1 (NBP1-31546), Nox4 (NB110-58851), b-tubulin (NB600-936). Data were analysed using two-tailed Student's t-tests. Bars represent means+/-SEM from at least five independent experiments. p

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Immunohistochemistry-Paraffin: NOX1 Antibody [NBP1-31546] - Human lung carcinoma. NOX1 antibody diluted at 1:500.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Flow Cytometry: NOX1 Antibody [NBP1-31546] - Nox-1 is present at the surface of resting platelets. Human platelets (4 x 10^8 cells/mL) were isolated from whole blood and incubated with the Nox-1 antibody or Rabbit IgG control at 1:250 dilution for 30 minutes at room temperature. FITC-tagged anti-rabbit (secondary antibody) was added at 1:100 dilution and incubated for an additional 30 minutes. Platelets were then diluted 1:25 v/v in Tyrodes buffer and read using a BD Accuri flow cytometer. Image from verified customer review.