F54379

antibody from NSJ Bioreagents

Targeting: POLG POLG1, POLGA

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

F54379

Provider

NSJ Bioreagents

Product name

POLG Antibody

Antibody type

Polyclonal

Description

Antigen affinity purified

Reactivity

Human, Mouse

Host

Rabbit

Conjugate

Unconjugated

Vial size

0.08 µl, 0.4 µl

Storage

Aliquot the POLG antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of human HepG2 cell lysate with POLG antibody. Predicted molecular weight ~140 kDa.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of mouse cerebellum lysate with POLG antibody. Predicted molecular weight ~140 kDa.

Immunocytochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Immunofluorescent staining of fixed and permeabilized human HeLa cells with POLG antibody (green) and DAPI nuclear stain (blue).

Immunohistochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC testing of FFPE human skeletal muscle tissue with POLG antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.

Flow cytometry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Flow cytometry testing of fixed and permeabilized human CEM cells with POLG antibody; Blue=isotype control, Green= POLG antibody.