PA5-21314

antibody from Invitrogen Antibodies

Targeting: POLG POLG1, POLGA

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-21314

Provider

Invitrogen Antibodies

Product name

POLG Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Recommended positive controls: MCF-7, MDA-MB-231, Rat testis.

Concentration

0.67 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of DNA Polymerase gamma using 30 µg of MCF-7 lysate. Samples were loaded onto a 5% SDS-PAGE gel and probed with a DNA Polymerase gamma polyclonal antibody (Product # PA5-21314) at a dilution of 1:1000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of POLG was performed by separating 30 µg of various whole cell extracts by 5% SDS-PAGE. Proteins were transferred to a membrane and probed with a POLG Polyclonal Antibody (Product # PA5-21314) at a dilution of 1:1,000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

POLG Polyclonal Antibody detects POLG protein by western blot analysis. A. 50 µg Rat testis lysate/extract. 5% SDS-PAGE. POLG Polyclonal Antibody (Product # PA5-21314) dilution: 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of DNA Polymerase gamma in methanol-fixed HeLa cells using a DNA Polymerase gamma polyclonal antibody (Product # PA5-21314) at a 1:200 dilution.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of methanol-fixed HeLa, using DNA polymerase gamma antibody (Product # PA5-21314) at 1:200 dilution.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded human skeletal muscle, using DNA polymerase gamma (Product # PA5-21314) antibody (10 µg/mL). Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.