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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
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- Product number
- PA5-21314 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- POLG Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Reactivity
- Human, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.67 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of DNA Polymerase gamma using 30 µg of MCF-7 lysate. Samples were loaded onto a 5% SDS-PAGE gel and probed with a DNA Polymerase gamma polyclonal antibody (Product # PA5-21314) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of POLG was performed by separating 30 µg of various whole cell extracts by 5% SDS-PAGE. Proteins were transferred to a membrane and probed with a POLG Polyclonal Antibody (Product # PA5-21314) at a dilution of 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- POLG Polyclonal Antibody detects POLG protein by western blot analysis. A. 50 µg Rat testis lysate/extract. 5% SDS-PAGE. POLG Polyclonal Antibody (Product # PA5-21314) dilution: 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of DNA Polymerase gamma in methanol-fixed HeLa cells using a DNA Polymerase gamma polyclonal antibody (Product # PA5-21314) at a 1:200 dilution.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of methanol-fixed HeLa, using DNA polymerase gamma antibody (Product # PA5-21314) at 1:200 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human skeletal muscle, using DNA polymerase gamma (Product # PA5-21314) antibody (10 µg/mL). Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
