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- References [3]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- ABIN954236 - Provider product page
- Provider
- antibodies-online
- Product name
- anti-Polymerase (DNA Directed), gamma (POLG) (C-Term) antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Vial size
- 0.1 mg
Submitted references Role of histidine 932 of the human mitochondrial DNA polymerase in nucleotide discrimination and inherited disease.
Polymerase γ gene POLG determines the risk of sodium valproate-induced liver toxicity.
Five mutations of mitochondrial DNA polymerase-gamma (POLG) are not a prevalent etiology for spontaneous 46,XX primary ovarian insufficiency.
Batabyal D, McKenzie JL, Johnson KA
The Journal of biological chemistry 2010 Oct 29;285(44):34191-201
The Journal of biological chemistry 2010 Oct 29;285(44):34191-201
Polymerase γ gene POLG determines the risk of sodium valproate-induced liver toxicity.
Stewart JD, Horvath R, Baruffini E, Ferrero I, Bulst S, Watkins PB, Fontana RJ, Day CP, Chinnery PF
Hepatology (Baltimore, Md.) 2010 Nov;52(5):1791-6
Hepatology (Baltimore, Md.) 2010 Nov;52(5):1791-6
Five mutations of mitochondrial DNA polymerase-gamma (POLG) are not a prevalent etiology for spontaneous 46,XX primary ovarian insufficiency.
Tong ZB, Sullivan SD, Lawless LM, Vanderhoof V, Zachman K, Nelson LM
Fertility and sterility 2010 Dec;94(7):2932-4
Fertility and sterility 2010 Dec;94(7):2932-4
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Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Western blot analysis of POLG Antibody (C-term) Cat.-No AP53379PU-N in mouse cerebellum tissue lysates (35ug/lane). This demonstrates the POLG antibody detected the POLG protein (arrow).
Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Confocal immunofluorescent analysis of POLG Antibody (C-term) Cat.-No AP53379PU-N with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).
Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Formalin fixed and paraffin embedded human skeletal muscle reacted with POLG Antibody (C-term) Cat.-No AP53379PU-N followed by peroxidase conjugation of the secondary antibody and DAB staining.
Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Flow cytometric analysis of CEM cells using POLG Antibody (C-term) Cat.-No AP53379PU-N (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
