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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- PPS028 - Provider product page
- Provider
- R&D Systems
- Product name
- GABA-A R alpha 6 C-Terminus Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Human, mouse, rat, and bovine ~57 kDa GABAA R alpha 6 subunit, C-Terminus
- Reactivity
- Human, Mouse, Rat, Bovine
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 uL
- Storage
- For long-term storage,
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Detection of GABAA R alpha 6 C-Terminus by Western Blot. Western blot of forebrain lysates from wild type and alpha 6 knockout animals, using approximately 5 - 7 μg of tissue per lane. Blots were incubated overnight at 2 - 8° C with anti-GABAA R, alpha 6 subunit, C-Terminus diluted 1:1000. The antibody labeled the ~57 kDa alpha 6 subunit of the GABAA R in the wild type (control) but not in the alpha 6 knockout animals.
