AP32492PU-N

antibody from Acris Antibodies GmbH

Targeting: GLRA3

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

AP32492PU-N

Provider

Acris Antibodies GmbH

Product name

anti Glycine receptor alpha-3 (C-term)

Antibody type

Polyclonal

Antigen

KLH conjugated linear peptide selected from residues surrounding the C-terminus of Human Glycine Receptor Alpha 3 subunit.

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

0.1 mg

Concentration

0.5 mg/ml

Western blot

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Western Blot Analysis (Representative lot data):Fetal Human brain lysate was resolved by electrophoresis, transferred to PVDF and probed with anti-Glycine Receptor Alpha 3 subunit. Proteins were visualized using a donkey anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates Glycine Receptor α3 subunit (48kDa).

Immunohistochemistry

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Immunohistochemistry Analysis: Figures 1+2: Representative positive and background staining patterns on formalin-fixed, paraffin-embedded in Mouse spinal cord. Tissue pretreated with citrate pH 6.0 using antigen retrieval techniques. Antibody diluted to 1/100-1/500 and detected using IHC Select HRP/DAB detection kit protocol. Immunoreactivity is seen as strong staining in Mouse spinal cord. Figure 1 (Low Magnification). Figure 2 (High Magnification).Figures 3 + 4: Representative positive and background staining patterns on formalin-fixed, paraffin-embedded Mouse eye. Tissue pretreated with citrate pH 6.0 using antigen retrieval techniques. Antibody diluted to 1/100-1/300 and detected using IHC Select HRP/DAB detection kit protocol. Immunoreactivity is seen as strong staining in Mouse eye tissue. Figure 3 (Low Magnification). Figure 4 (High Magnification).