Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- BS2059 - Provider product page
- Provider
- Bioworld Technology, Inc
- Proper citation
- Bioworld Technology Cat#BS2059, RRID:AB_1662310
- Product name
- GCKR (R227) polyclonal antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide, corresponding to amino acids 196-245 of Human GCKR.
- Description
- Glucokinase (also designated hexokinase IV or GCK) plays a key role in the regulation of glucose-induced insulin secretion. GCK is expressed in pancreatic beta cells, where it functions as a glucose sensor, determining the ¡ùset point¡ì for insulin secretion. GCK is also expressed in the liver, where it catalyzes the first step in the disposal of glucose. A lack of glucokinase activity leads to reduced insulin secretion and hyperglycemia and has been implicated as a cause for maturity onset diabetes of the youth (MODY). Heterozygous point mutations in the gene encoding GCK have been detected in individuals suffering from MODY. GCK is regulated by GCKR (glucokinase regulatory protein). GCKR is a 68 kDa protein which is expressed in pancreatic beta cells and in the liver.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100ul
- Concentration
- 1 mg/ml
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
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Supportive validation
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- Bioworld Technology, Inc (provider)
- Main image
- Experimental details
- Western blot (WB) analysis of GCKR (R227) pAb at 1:500 dilutionLane1:A549 whole cell lysateLane2:Raw264.7 whole cell lysateLane3:PC12 whole cell lysate