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- Antibody Data
- Antigen structure
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- Western blot [1]
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- Product number
- BS4858 - Provider product page
- Provider
- Bioworld Technology, Inc
- Product name
- PLD2 (phospho Y169) polyclonal antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic phosphopeptide derived from human PLD2 around the phosphorylation site of Tyrosine 169.
- Description
- Phosphatidylcholine phospholipase D1 and D2 (PC-PLD1 and PC-PLD2) are phospholipid-specific phosphodiesterases that hydrolyze phosphatidylcholine. Unlike PC-PLD1, which associates with secretory granules, PC-PLD2 localizes to the plasma membrane, where it is implicated in the formation of endocytotic vesicles. Both PC-PLD1 and PC-PLD2 coordinately regulate macrophage phagocytosis. PC-PLD activity in mammalian cells is transiently stimulated upon activation by G protein-coupled and receptor tyrosine kinase cell surface receptors. In addition, tubulin binding to PC-PLD2 inhibits muscarinic receptor-linked PC-PLD2 activation. PC-PLD2 also enhances PKCzeta activity through direct interaction in a lipase activity-independent manner.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100ul
- Concentration
- 1 mg/ml
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
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Supportive validation
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- Bioworld Technology, Inc (provider)
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- Experimental details
- Western blot (WB) analysis of p-PLD2 (Y169) pAb at 1:500 dilutionLane1:HEK293T cell lysate treated with TNF¦Á(20ng/ML,15mins)Lane2:NIH-3T3 cell lysate treated with TNF¦Á(20ng/ML,15mins)Lane3:H9C2 cell lysate treated with TNF¦Á(20ng/ML,15mins)
